... aspens, and cottonwoods) are model angiosperms for molecular genetic ... strategy is to devise forward genetic screens using more ... Southern-blot analysis indicated that gene ...
Southern blot of Hind III digested genomic DNA from common chimpanzees ( Pan troglodytes ), bonobo ( Pan paniscus ), human ( Homo sapiens ) and orangutan ( Pongo pygmaeus ).
100 EXPERIMENT NINE: SOUTHERN BLOT The objectives of this experiment are to: (1) restrict ... face-up inside the UV cross-linker and expose to appropriate amount of UV light ...
Bio 181 Internet-based exercise: Week 7 DUE: Monday, April 23rd Southern Blotting #1. Suppose the map below represents linear DNA "X". You perform a Southern blot ...
Screen ES cells by Southern Blot Digest DNA in 96-well plate To each well add: 4ul 10Xbuffer 4ul Enzyme 0.4ul Spermidine(0.4M) 31.6ul H 2 O 37‡C 19h, then add 4ul ...
www.flemingtonlab.com Southern-blot Analysis Protocol I. Salt transfer - Cut membrane (GeneScreen Plus, NEN Research Products, Cat# NEF-976) to size of gel.
Analysis of nucleic acids Analysis of Nucleic Acids 1 Southern Blot Digest approximately 15µg of genomic DNA sample with appropriate restriction enzyme and ...
Southern Blot Hybridization blotting 1. Run gel to desired distance. 2. Visualize gel on UV box. Note the marker bands by: a) using a fluorsecent ruler and ...
Discriminatory Power of RAPD,PCR-RFLP and Southern Blot Analyses of ureCD or ureA Gene Probes on Helicobacter pylori Isolates Stella Smit ha, *, Franck Cantet b, Fa ...
Making Solutions for Southern Blot Analysis) •Sheared and denatured salmon sperm DNA •100X Denhardt's ... Important: When using this labeling method on Odyssey, it is ...
Southern Blot Protocol – Gause Laboratory Page 2 of 2 RUNNING THE GEL 1. Make 350 mL of a 1% agarose gel as follows: 3. 5 g agarose in 300mL ddH2O, stir briefly ...
1 of 5 12/99 Nucleic Acid Detection, Southern Blot Southern-Light Allefs, J. J., Salentijn, E. M., Krens, F. A. & Rouwendal, G. J. (1990). Optimization of non-radioactive
Southern Blot Protocol DAY 1: Digestion of DNA - Digest 10 µg of genomic DNA with 1 µl of the appropriate enzyme making the final volume 40 µl.
PROBE LABELLING AND BLOT PROCESSING To detect specific restriction fragments on the genomic Southern blot you prepared earlier, you will radioactively label a ...
Southern Blot Hybridization and Detection I. Prehybridization and Hybridization A. Hybridization Buffer a. 50% (v/v) formamide ... for 15 minutes at 68°C. 5.
Southern Blot Protocol 1) Digest genomic DNA at 37 C overnight. a) 10 g DNA with 5 Units restriction enzyme / 1 g DNA Sample Amount to Add (Concentration L)
FMR1 Methylation PCR: Eliminating the need for Southern Blot testing Elaine Lyon, PhD, FACMG Associate Professor of Pathology University of Utah School of Medicine
Filename: Southern Blot 0807222.pdf
Typed 22 July 2008 original 3/1/91 SOUTHERN BLOT (Church, G.M., Gilbert, W. P.N.A.S. 81: 1991-1995, April 1984) 1. Run restricted DNA on agarose gel as usual. 1 …
Filename: Typing Mice by Southern Blot in 2 Days.pdf
Typing Mice by Southern Blot in 2 Days Tail DNA (The protocol of the awesome Luis) ... (5 U) 4 ?l 32P-dCTP (3000 Ci/mmol), 3 ?l is fine in times of shortage.
Figure 1. Ethidium bromide staining of 1% agarose gel before capillary transfer of DNA to Nytran nylon Membrane for Southern blot hybridization analysis.